UCT Inc., is highly regarded by researchers in the area of biomechanical analysis for the manufacture and production of the very finest specialty chemicals including silanes and siloxanes. In a new paper by Prof. J.Hickman et al., NanoScience Technology Center, University of Central Florida (Journal of Biomaterials (10.1016/j.biomaterials.2017.01.005)), UCT’s specialty chemical trimethoxysilylpropyldiethylenetriamine (DETA, T2910KG) was employed to produce a film on microscope slides. These slides were then used for the induction of human intrafusal skeletal muscle fibers from satellite cells and their synaptic connection with human stem cell-derived sensory neurons in a defined in vitro system.
Muscle spindles are sensory organs embedded in the belly of skeletal muscles that serve as mechanoreceptors detecting static and dynamic information about muscle length and stretch. Through their connection with proprioceptive sensory neurons, sensation of axial body position and muscle movement are transmitted to the central nervous system. Impairment of this sensory circuit causes motor deficits and has been linked to a wide range of diseases. To date, no defined human-based in vitro model of the proprioceptive sensory circuit has been developed. This present study developed a human-based in vitro muscle sensory circuit utilizing human stem cells.
A serum-free medium was developed to drive the induction of intrafusal fibers from human satellite cells by actuation of a neuregulin signaling pathway. Both bag and chain intrafusal fibers were generated and subsequently validated by phase microscopy and immunocytochemistry. When co-cultured with proprioceptive sensory neurons derived from human neuroprogenitors, mechanosensory nerve terminal structural features with intrafusal fibers were demonstrated. Most importantly, patch-clamp electrophysiological analysis of the intrafusal fibers indicated repetitive firing of human intrafusal fibers, which has not been observed in human extrafusal fibers. This study demonstrates why researchers performing intricate yet delicate studies requiring the finest,specialty chemicals of the highest quality turn to UCT.