UCT Inc., is known around the world for the manufacturing of the very finest in specialty chemicals including (N-trimethoxysilylpropyl)-polyethylenimine. These compounds are regularly employed by researchers in fields such as the production of methacrylate-based monoliths for highly efficient separations. Monolithic materials are versatile adsorbents widely employed in separation science, sample preparation and as supports for flow through applications. In a recent paper published in Journal of Chromatography A by P. Simone et al., (Sapienza Università di Roma, Italy (http://dx.doi.org/10.1016/j.chroma.2016.11.039)) capillary methacrylate-based monoliths were prepared for the high performance liquid chromatography (HPLC) separation of both small molecules and large biomolecules. An efficient grafting from/to synthetic approach was adopted introducing a network of activated sites in the inner wall surface using the new silanization agent (N-trimethoxysilylpropyl)-polyethylenimine obtained from UCT.

Copolymerization of lauryl methacrylate monomer and 1,6-hexanediol dimethacrylate cross-linker in the presence of porogenic solvents was obtained under continuous gamma -ray exposure with high conversion yield. The morphology and porous structure of the resulting monoliths have been investigated by Scanning Electron Microscopy (SEM) and 1H NMR cryo-porosimetry. By chromatographic investigation, the new capillary columns attested high kinetic performance (with efficiency larger than 100,000 theoretical plates per meter for small molecules at optimum mobile phase linear velocity of about 0.5 mm/s) and also excellent mechanical stability and repeatability. The new methacrylate-based monolithic capillary columns have been successfully employed for efficient reversed-phase separation of intact proteins and peptides. This article demonstrates why when world class researchers need the very finest of silanization reagents the turn in the first instance to UCT.